Meet the DBI users
DBI video series about DBI users. Researchers affiliated at different Danish institutions shared their bioimaging research with our community; highlighting how important is for their work to use DBI core facilities in order to pursue their research, how are they using the services we offer, both equipment and access to the knowledge and expertise of the core facility staff.
Check out our latest interview with Rikke Agerskov, a PhD researcher the Department of Science and Environment at Roskilde University (RUC)
Meet the DBI users! In this issue, we interview Rikke Agerskov, a PhD researcher the Department of Science and Environment at Roskilde University (RUC). Rikke is working on an exciting project utilizing the Olympus scanning confocal microscope at RUC, focusing on how the nervous system affects the embryonic development of the pancreas. Her work aims to make a significant impact on society by advancing basic science through identifying new neuronal-epithelial signaling mechanisms. Understanding the link between the peripheral nervous system and the pancreas will help improve stem cell protocols for producing beta cells and islet organoids, leading to new treatment options for diabetes. Watch this short video to learn more about Rikke’s research journey, her current interdisciplinary project, and how access to advanced microscopy technologies at RUC has empowered her to perform high-resolution imaging of embryonic pancreatic tissue.
If you’re a researcher interested in using the facility for your research, please reach out to Pia Nyeng for more information on accessing the core facility at RUC. Microscope-core.ruc.dk
Meet Istvan Balint Szücs, using DBI-INFRA to investigate spontaneous miscarriage! In this issue, we interview Istvan Balint, a PhD researcher from the Center for Chromosome Stability, Michael Lisby group, at the Københavns Universitet - University of Copenhagen.Istvan is using an automated High Content Screening (HCS) spinning disc confocal microscope at the Center for Advanced Bioimaging (CAB) to perform imaging of sporulating fluorescent yeast tetrads, in order to study oocyte aneuploidy linked to meiotic recombination and non-disjunction (the failure of the chromosomes to separate). This condition can contribute to chromosomal abnormalities and is associated with an increased risk of pregnancy loss or miscarriage. “About 30% of spontaneous miscarriage in women are associated to genetical events that we do not really know what the reason is behind, and we still do not understand the key players on this”, stress Istvan.
In this edition, we interview Serhii Kostrikov, a postdoctoral researcher from the Center for Auditory Neuroscience at DTU Health Tech, who has been using the ZEISS Microscopy Light Sheet 7 at the DBI Core Facility for Integrated Microscopy, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, UCPH, University of Copenhagen. His areas of expertise are neurodegeneration, brain vasculature pathology, cancer, and drug delivery using 3D deep microscopy and machine learning-based image analysis. Serhii has used light sheet microscopy for different projects; from brain tumor vasculature and neurodegeneration, to hearing loss and its connection to dementia. Watch this video to learn more about Serhii’s research, and about how access to microscopy technologies at CFIM empowered him to perform 3D imaging of various organs. As Serhii stresses, access to technical expertise from the core facility staff has been essential for his work; from sample preparation to image analysis guidance and support. The Light Sheet 7 was financed by the Novo Nordisk Foundation: Application Calls and Grants. Serhii's work is funded by the William Demant Foundation.
Meet the DBI-INFRA users! Check out our first interview with Associate Professor Kasper Urup Kjeldsen from the Department of Biology at Aarhus University who is using our infrastructure at the Aarhus BioImaging Core Facility for his research. He is hunting for a special type of Archaea (Asgard archaea) that inhabited the muddy sediments of Aarhus Bay. “I use the LSM800 confocal laser scanning microscope from Zeiss for my project because I need good resolution as we image tiny cells. It is an excellent confocal microscope with great resolution, and in addition to that it has an Airyscan super resolution module that is very straight forward to use, and allows for the higher resolution needed to image small bacteria”, explains Kasper.
Meet our users! Marie Bølling Kongsted - CFIM user
Marie Bølling Kongsted is a biologist, and her main research focus is to understand the formation and function of the heart. For her research, she is using zebrafish to study cardiac diseases using different genetic mutants. Zebrafish cardiovascular system shares many similarities with that of humans. Marie´s research provides valuable insights into the genetic and molecular mechanisms underlying cardiac diseases that potentially could lead to the development of new therapies and treatments for the patients.
Marie has used the Core Facility for Integrated Microscopy (CFIM) to pursue her research goals. She used a broad range of technologies, including slide scanners, spinning disk, laser-scanning microscopy, and recently, with the Zeiss LS7 microscope, she was able to acquire full 3D hearth imaging at the latter stage of the zebrafish development, which was not possible with conventional confocal and wide-field techniques.